ALL THE BEST!

Best wished for the future, T08 crowd!

You were definitely one of the hardest working sections that I've ever seen. Keep it up, but also enjoy the rest of your Summer!

Cheers

Pam

(my email: kalas[at]zoology.ubc.ca)

THURSDAY

Thursday's tutorial will be mainly a review.

Please come prepared...bring all the questions you have about BIOL335!

* operons and bacterial gene regulation

* restriction mapping

* cloning (general approaches)

* techniques (Southers, northerns, transformations, selections, PCR, RT-PCR, footprinting, colony lift hyb., indicator-based assay systems, chromosome walking...)

* eukaryotic gene regulation and development

* genomics (molecular markers, contigs, sequencing of whole genomes...)

* GPCRs

Happy studying!

ABOUT DROSOPHILA DEVELOPMENT....

PLEASE POST HERE ALL YOUR QUESTIONS REGARDING DEVELOPMENT. A FEW NOTES FOLLOW....(they're just some quick notes that I made some time ago, and do not replace, by any means, your class notes or the textbook, or any other course materials!)

TRANSPOSABLE ELEMENTS

Today we talked about:

- the differences between retrotransposons and 'regular transposons'

- the two main components of a 'regular' transposable element (not a retrotransposon), and what happens if one of them is mutated

- hybrid dysgenesis in flies (P elements).

We practiced:

- predicting the outcomes of crosses where one or both parents carry one or more transposable elements;

- diagnosing the presence of a transposable element in a gene.

If you have any questions regarding these topics, please post them here!

If you have questions regarding how to clone a gene taking advantage of a P element, you can also post it here, but please start the comment with "Cloning and P elements question".

Have a good weekend!

Pam

PS: more about P elements here.

GENERAL NOTICES

1- I have added some quick notes on chromosome walking and on the use of P elements (here).

2- If you were in the 8am tutorial in BIOL334, ave a look at the meiosis gallery and see if you can find the piece of art that you and your group created...let me know which one it is and whether you give me permission to show it to others (your drawings are copyrighted!) and whether you want your name associated with your drawing or not.

Thank you!

(You can also suggest a title and a caption for the image...)

GENOMICS AND POSITIONAL CLONING

Please post all your questions about positional cloning, chromosome walking, clones alignment, sequencing of entire genomes, etc, here!

I have added a couple of questions and pieces of notes about genomics-related topics to the 'questions and answers' page.

IMPORTANT NOTICES

No tutorial on Monday!

Lac operon BIOL335 conventions: today (Friday) I told you that in the presence of glucose and inducer you do, definitely, get B-gal production, although in lecture, you learned that you don't. After talking to Craig, we got it sorted out-and he clarified it for you in class.

The miscommunication was about what we call '-': for 335 our definition of '-' will extend to the amount of expression that we get in the presence of glucose and inducer, or with a mutant crp gene.

For your exams, the -, + and ++ will be clearly defined in the question.

In general we'll say that:

mutations in the promoter, in the lacZ, in the crp gene, and lacIs mutations will give us a "-" in all cases

a WT strain grown without inducer will give us a "-"

a WT strain grown in the presence of inducer and glucose will give us a "-"

a lacI- strain grown in the absence of glucose will always give us "++"

a lacI- strain grown in the presence of glucose will give us a "-" (same as WT with inducer and glucose)

a lacOc strain in the presence of glucose will give us a "-"

a lacOc strain in the absence of glucose and presence of inducer will give us a "++"

a lacOc strain in the absence of glucose and inducer will give us a "+"

lacOc wins over lacIs....but adding inducer does not make a difference:

lacOc lacIs in the absence of glucose is always "+"

lacOclacIs in the presence of glucose is always "-"

WANT TO KNOW MORE ABOUT THE LACTOSE OPERON? Here is a recent review. If you want to see how they 'figured out' the basic idea of the lac regulatory system, check out the first reference in the review paper!